how to disable USB port

Mostly Intel D845GVSR, Intel D845PESV and Intel D845EBG2.

What do you mean by update the BIOS for motherboard? How to do that?

The BIOS disable is likely for USB in DOS mode so that a USB Keyboard or Mouse will work before Windows starts.
Windows is seeing the USB chip and installing the driver.
Disable through the device manager in control panel.
If you need to get hard core pop-rivet or glue a plate over the ports or clip the leads to the jacks.

You can also try DeviceLock from http://www.protect-me.com/download.html

You could buy lots of rivets for $2500.

PCBONEZ, how to disable through device manager? I did remove all the USB in the device manager, but they will install back on next reboot.

No not 'remove' --> 'disable'..
It's different.

It varies a little by Windows version but it's close enough to figure it out.
You said Win98 so...

In Device Manager go to "Universal Serial Bus Controllers"
Expand it and you will see the USB chip and USB hub listed.
One at a time highlight each of those and click on 'properties'.
At the bottom of the properties pop-up window there's a box that's titled "Device Usage".
Put a check in the box that says "Disable in this Hardware profile".

Now what happens when you reboot is windows already knows it's there so it won't install the driver again but Windows won't turn on the function. ~ Dead USB ports.

The only problem with this is if there's anyone in the office that's familiar with PC's they may figure out what you did and re-enable the ports.

If you really wanna get nasty I can tell you how to rewire the ports for 12 Volts instead of 5 Volts.
Meltdown 2 or 3 thumb drives and word will get around the office...
.
.

Yes, i understand what you mean. That's why i was thinking if i could disable in the BIOS, and i can set a password that only i can go into the BIOS, then i can disable the USB port for good. But if i could only disable in the device manager, then this problem occur.

I would like to know how to rewire the ports. But just to confirm, can I undo the rewire, and reuse back the USB port later if I want?

Pin 1 ... +5 volts dc ........ Red
Pin 2 ... -Data ............White
Pin 3 ... +Data ........... Green
Pin 4 ... Ground (for the 5 volts dc.) ....... Black

Here's and image so you can see the pin arrangement.
http://www.starmount.co.uk/usb_skta.gif

If you disconnect pin 1 then devices that rely on USB power to operate (like a thumb drive) won't power up.
( And if you then run a jumper wire to a 12 volt fan header or a spare PSU 4 pin... ... Sizzle - Pop. .. )

If you disconnect either Pin 2 or 3 then data won't go.

There are several methods you might try depending on the specific jack in question, your skill level, and your comfort zone.

1 - You could simply unsolder the whole jack and remove it. (Could easily be put back later.)
~ Most difficult now. May be easier to restore the board later than other options.

2 - The tin cover on those jacks (the little metal cube they are in) is normally removable with some minor effort. This usually gives access to the conductors inside. Simply snip the one you want cut with some small electronics nippers. Bend it slightly so there's no accidental contact. To restore later, bend the ends back in firm contact and apply a smidgen of solder.

3 - (Easiest if it will work on the particular board.)(Actually with this method it would be easy enough to cut continuity to all or most of the pins.)(The problem with this method is depending on the board's layout all of the conductors might not be there. Some may be on the other side and under the jack.)
On the underside of the motherboard you will see the 'solder traces' running to the solder points. You should do continuity check from the plug side of the jack to the solder point to verify you have the correct one.
Take an exacto knife and remove a 1/16th to 1/32nd of an inch band across the line you want cut. This is repaired later by cleaning the coating off the trace on either side of the cut down to the copper and then bridging the gap with a 1/8th to 1/4 inch length of small diameter copper wire (uninsulated) and some solder. The wire out of a piece of phone line works great for this. You only need a tiny amout of solder and use a low heat iron. (15 to 20 Watts). Too much heat and the trace will 'lift' which you would have to fix by cleaning it back to the good (un-lifted) area and using a longer piece of wire.
~ This sounds rough but it's really very easy both now and to restore later.
You could practice the technique on a dead video card, mobo, whatever you have around.
.

~~~ Use the smallest diameter solder you can find for this. It melts quicker -> less heat to the traces.

Thanks for the information PCBONEZ, but i still hope to find a simpler way to do this. I'm talking about 50 pcs here in the office. So i don't think open up the computer, and solder to rewire is the way. Actually I wish to only disable the port, but possible to re-open in the future.

I just don't understand why I can't do it in the BIOS?? I can disable the floppy in BIOS, but USB cannot. Is it something I left out or what? I goes into the BIOS. Inside BIOS there are MAIN, Advanced, Security, Power, Boot, Exit menu. I goes into Advanced. Inside Advanced there are PCI configuration, peripheral configuration, boot configuration, IDE configuration, Event log configuration, video configuration, chipset configuration and USB configuration. I goes into USB configuration. Inside USB configuration there are Hi-speed USB and Legacy USB support. I disabled both. I can't find any other USB except in the Boot menu, there is a USB boot. I also disable that. Where else?? I have set the password so if successfully disable in the BIOS, no one will be able to reopen except me.

i raise this point to 100. Please help!! Thank you!!

Best I can explain it. And it's only a guess...
USB came out after Win98.
Win98 can handle USB due to a patch that came later on.
Win98 doesn't check the BIOS for USB during start-up.
It checks for a chip after that phase of start-up. (Like modem detection.)
If it finds one it puts in the driver for it.

You might have some success if you go into properties and drivers and write down the files being used for USB drivers, then delete those files from the system. If you also 'disable' the device before you reboot then hopefully 98 won't ask for the Win98 CD everytime you start up there after.

Honesty in your situation I think the best solution is to glue or rivet a small metal plate over the port hole in the I/O sheild.
~ Or just fire the next person caught with a thumb drive.

I like the last suggestion, but unfortunately i'm not the boss. :-)

actually yesterday i did found some information in disabling the USB in registry, but that is for windows 2K and above, not 98. :-(
http://support.microsoft.com/default.aspx?scid=kb;en-us;823732