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How to split a Wave file (Part Two)

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Ok I will look this over and I will fill in some blanks.  I had another question that if you know the musical notes of a song, is it possable to isolate each note, assuming the wav file as one instrument.  Can you generate a list of notes (see below).  I realize that no two notes are the same, however I believe that I can compair them.

1/2 note A# sounds like (wav file)
1/8 note A# sounds like (wav file)
1/2 note Ab sounds like  (wav file)

I believe another 500 pt Question is in order.  Also I have several other Questions (clearly stated , I think) that you may know the Answer to.  Check my profile

Also does wav file format only support two channels

Thanks for your help:)