After a bit more investigation I think I've found the problem.....not sure how I missed it before.
Looks like the severs had hotfixes applied in the domain and then all rebooted at the same time so the DC wasn't up to authenticate the user ID and flagged it as invalid.
I'll get the reboot schedule changed first and if the problem still persists I'll look further at the App Pool as suggested.
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by: crokeefe28Posted on 2009-02-26 at 08:27:27ID: 23746382
First and foremost...in IIS 6 there is no real benefit in running the application pool under a domain service account. The NETWORK SERVICE or ISR account is just fine and has less of a chance in having permissions to network resources than a domain account. If you need access to resources in the application, such as a network share, this can be setup in the IDENTITY Element within the web.config and then encrypted so no one can see it. In IIS 7, this has changed and Application Service accounts are then ultilized. I would delete and recreate the App Pool using the NETWORK SERVICE account (default in Application Isolation Mode for IIS6)