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hamisherskineFlag for Yemen

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How many workstations can you have on a W2000 Server domain without overloading the server or network?

I have a single domain controller running W2000 Server. When I increased the number of workstations from 80 to 90, I started to get problems of workstations losing their connection to the domain. I wonder whether I may be running into a limit of how many workstations my server can handle, or perhaps how many my Dlink hubs can handle. My main hub is a 24 port 100 Mbps Dlink, to which 13 other Dlink hubs are attached, serving the workstations in various locations. Hub to hub connections are with CAT6 cable, hub to workstation cables are CAT5e. Any thoughts?
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Brian Pierce
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Many thanks