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Slow internet connection using NAT overload.

I have 50 users in my environment who all translate to a single public IP address using NAT overload on a pix 515.  Usually when testing throughput on speedtest.net, my users were getting  80mb down and 70mb up.   A few days ago i noticed that the numbers dropped significantly  to 35/25.  I was able to narrow down the problem to pix nat overload function by assigning a static one to one address to an inside client and the clients speed were restored to 80/70.  I checked xlate and found aprox. 600 translations - after doing clear xlate , the problem did not resolve.  I am stumped and appreciate anyone jumping on this.  
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UPDATE:  Oddly enough I have 1 Mac and 1Vista machine on the network and both are not effected by this plague.  Makes me think that this might be a bad Microsoft Update....
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It would not be the first time a bad Msoft update killed something.
XP SP3 is supposed to have the same IP stack as Vista. Are any of the affected machines SP3?
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