Membership credential verification failed.

I have two webservers, one is a backup just waiting in the wings if the other fails, they both run an identical website.  The website runs asp and sql express,   Now out of no where we cant login to our form on either machine.   It doesnt make any sense but as of yesterday we cant log in to the form using either machine.  Below is the event that pops up in even viewer, any ideas ??
 
We are running windows 2003 Enterprise on both machines.


 
 
 
ASP.Net 2.0.50727.0 Web event 1315 N/A
 
 
Event code 4006
 
Membership credential verification failed.
 
level9techAsked:
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spprivateConnect With a Mentor Commented:
See if somebody has enabled anonymous login property in Virtual directory.(Directory Security Tab)
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level9techAuthor Commented:
no not in the virtual directory..

in the directory security tab in IIS anonymous access is enabled
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spprivateCommented:
Yeah,thats what.If you enable that it wont autheticate against active directory.
was this working before with the anonymous access enabled.I am sure not
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level9techAuthor Commented:
we are not trying to authenticate with active directory. it a just a form if that makes any sense
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level9techAuthor Commented:
we are getting event 1309 from event code 3005 as well
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spprivateCommented:
I mean was that anonymous enabled earlier or somebody changed it now?
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