How to clear .mdf file read only feature

I developed web application using webservices.Services independenty working properly.
when the services are used in webapplication working properly some times.the actual problem
is when project is deployed on other system independently services ok. but using web application login process is also ok. when i tried modify or delete or insert data using web service into sqlserver i.e .net 2005 , iis (environment).. it displays message insertion failed beceause .mdf file is readonly.

i cleared read only attributed of application folder and inside files also.

i setted write  option (chekc box) of application folder using propeties option from inetmgr (iis)
i used vs.net 2005 inbuilt sqlexpress- web application
mallik_pavuluriAsked:
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