Intranet site suddenly not allowing login

I have a program that runs through the web on my server.  A couple of weeks ago it stopped allowing anyone to login.  It gives this error (even trying from the server itself) HTTP Error 401.1 - Unauthorized: Access is denied due to invalid credentials.  I did not knowingly do anything to change iis or the program.  I did realize this morning that I ran a script to try to set the default ftp domain on another server a few weeks back.  Could this have affected the logon procedure for an iis website on another server (if it changed something domain wide)?
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hindsightAsked:
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hindsightConnect With a Mentor Author Commented:
Which do I try?  I have a single application pool running with the Network service.  I have never needed credentials before.  It should be the login page of the software which has it's own usernames and passwords.
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halejr1Commented:
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halejr1Commented:
is it a login page from the software that's popping up?  I guess I don't understand.. :(
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