ascii charact coming back from database.

Is this data coming like that from db or are you doing any sort of HtmlEncode or alike with it before displaying?

Try this:

 txtProblem.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["ProblemNote"].ToString());

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You need to include the System.Web namespace to use the above.

if (TicketDS != null && TicketDS.Tables.Count != 0 && TicketDS.Tables[0].Rows.Count > 0)
            {
                txtActionTaken.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["ActionNote"].ToString());
                TicketNumber.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["TicketNumber"].ToString());
                txtClosedBy.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["AgentClosed"].ToString());
                txtCreatedBy.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["AgentCreated"].ToString());
                txtDateAssigned.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["DateAssigned"].ToString());
                txtDateClosed.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["DateClosed"].ToString());
                txtDateCreated.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["DateCreated"].ToString());
                txtDateFollowup.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["FollowupBy"].ToString());
                txtExternalTicket.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["ExternalTicket"].ToString());
                txtFollowupNotes.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["FollowupNote"].ToString());
                txtLastUpdate.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["DateLastModified"].ToString());
                txtProblem.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["ProblemNote"].ToString());
                txtShortDescription.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["ShortDescription"].ToString());
                txtSolution.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["SolutionNote"].ToString());
                txtSubmitVia.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["TicketApplicationType"].ToString());
                txtTrackIt.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["TrackIt"].ToString());
                txtUserID.Text = HttpUtility.HtmlDecode(TicketDS.Tables[0].Rows[0]["UserID"].ToString());
                uxLookupFieldTicketStatus1.DefaultValue = TicketDS.Tables[0].Rows[0]["TicketStatus"].ToString();
                uxLookupFieldTicketAgentFolowup1.DefaultValue = TicketDS.Tables[0].Rows[0]["AgentFollowup"].ToString();
                uxLookupFieldTicketType1.DefaultValue = TicketDS.Tables[0].Rows[0]["TicketType"].ToString();
                uxLookupFieldTicketServiceImpact1.DefaultValue = TicketDS.Tables[0].Rows[0]["ServiceImpact"].ToString();
                uxLookupFieldTicketLocationSIMs1.DefaultValue = TicketDS.Tables[0].Rows[0]["LocationSIMs"].ToString();
                uxLookupFieldTicketRequestType1.DefaultValue = TicketDS.Tables[0].Rows[0]["RequestType"].ToString();
                uxLookupFieldTicketPriority1.DefaultValue = TicketDS.Tables[0].Rows[0]["Priority"].ToString();
                uxLookupFieldTicketContactMethod1.DefaultValue = TicketDS.Tables[0].Rows[0]["ContactMethod"].ToString();
                uxLookupFieldTicketAssignedTo.DefaultValue = TicketDS.Tables[0].Rows[0]["AssignedTo"].ToString();
            }
           

is it ok for each textbox that is being assigned,

Thanks.