Impact of Updating Active Directory

We migrated from Windows 2000 Native Mode Active Directory to Windows Server 2008. On our domain controller, it shows Windows 2000 Native. We are currently not experiencing any problems with AD, but thought we should probably move it at least to Windows 2003 native mode. We have Win2003 and Windows 2008 servers.

Thoughts, suggestions?

Is this an after hours change or can I do it during the day?
Who is Participating?
definately after hours and with a system state backup (which has been verified).

provided you don't have any Windows 2000 DC's, it should be OK - but best be safe and do the above.
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