How should I partition, where should I install my OS?

I have three hard drives and one is a 1TB used for storage.  The other two are:

an older 250gb caviar:

http://www.wdc.com/en/products/legacy/Legacy.asp?r=8&Model=WD2500YD

and a relatively newer 500GB caviar black:

http://www.wdc.com/en/products/products.asp?driveid=552

I am going to reformat these drives, below are the speed tests.

QUESTIONS:

1) How should I hook these three drives into my MB across the 4 ports bank 0 and 1?  Does the order even matter?

2) Where I should put my OS.  I see two possible configurations:

A: Partition the 500 into a 100gb section for the OS and a 400gb for storage.  POSSIBLE PROBLEM: the read head will have to move from the OS portion to the storage portion thus slowing down access.
B: Use the 250gb drive as the dedicated OS drive.  POSSIBLE PROBLEM: Based on HD Tune it seems to be the slower drive.

THANKS!






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