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Check this Vmware KB.<br />
<a href="http://kb.vmware.com/selfservice/microsites/search.do?language=en_US&cmd=displayKC&externalId=1026661" rel="ugc nofollow">http://kb.vmware.com/selfservice/microsites/search.do?language=en_US&cmd=displayKC&externalId=1026661</a>
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Check this Vmware KB.
http://kb.vmware.com/selfservice/microsites/search.do?language=en_US&cmd=displayKC&externalId=1026661 [http://kb.vmware.com/selfservice/microsites/search.do?language=en_US&cmd=displayKC&externalId=1026661]

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There is a VM using one of the files on that datastore. probably a cd image still in use by a VM on that host. 
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There is a VM using one of the files on that datastore. probably a cd image still in use by a VM on that host.

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Receive this error message Call &quot;HostDatastoreSystem.Remov<wbr />eDatastore<wbr />&quot; for object &quot;ha-datastoresystem&quot; on ESXi &quot;192.168.x.x&quot; failed when deleting the datastore. Please help.
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Receive this error message Call "HostDatastoreSystem.RemoveDatastore" for object "ha-datastoresystem" on ESXi "192.168.x.x" failed when deleting the datastore. Please help.

Cannot Delete Datastore using vClient ESXi 4.1

Computer data storage, often called storage or memory, is a technology consisting of computer components and recording media used to retain digital data. In addition to local storage devices like CD and DVD readers, hard drives and flash drives, solid state drives can hold enormous amounts of data in a very small device. Cloud services and other new forms of remote storage also add to the capacity of devices and their ability to access more data without building additional data storage into a device.