Partner

Check the event log entries, run dcdiag and see what messages you are getting there too.

Do you have other domain controllers

Thanks

Mike

Check the 2000 box to see if there is a SYSVOL share.

Ran DCDIAG, seems related to SYSVOL.  see below, any thoughts?

Microsoft Windows 2000 [Version 5.00.2195]
(C) Copyright 1985-2000 Microsoft Corp.

C:\Documents and Settings\Administrator>dcdiag

Domain Controller Diagnosis

Performing initial setup:
   Done gathering initial info.

Doing initial required tests

   Testing server: Default-First-Site-Name\DC
      Starting test: Connectivity
         ......................... DC passed test Connectivity

Doing primary tests

   Testing server: Default-First-Site-Name\DC
      Starting test: Replications
         ......................... DC passed test Replications
      Starting test: NCSecDesc
         ......................... DC passed test NCSecDesc
      Starting test: NetLogons
         ......................... DC passed test NetLogons
      Starting test: Advertising
         ......................... DC passed test Advertising
      Starting test: KnowsOfRoleHolders
         ......................... DC passed test KnowsOfRoleHolders
      Starting test: RidManager
         ......................... DC passed test RidManager
      Starting test: MachineAccount
         ......................... DC passed test MachineAccount
      Starting test: Services
         ......................... DC passed test Services
      Starting test: ObjectsReplicated
         ......................... DC passed test ObjectsReplicated
      Starting test: frssysvol
         Error: No record of File Replication System, SYSVOL started.
         The Active Directory may be prevented from starting.
         There are errors after the SYSVOL has been shared.
         The SYSVOL can prevent the AD from starting.
         ......................... DC passed test frssysvol
      Starting test: kccevent
         An Warning Event occured.  EventID: 0x80000443
            Time Generated: 12/05/2010   13:58:05
            Event String: The ntdsConnection object
         An Warning Event occured.  EventID: 0x800004F1
            Time Generated: 12/05/2010   13:58:05
            Event String: The attempt to establish a replication link with
         An Warning Event occured.  EventID: 0x800004F1
            Time Generated: 12/05/2010   13:58:05
            Event String: The attempt to establish a replication link with
         An Warning Event occured.  EventID: 0x800004F1
            Time Generated: 12/05/2010   13:58:05
            Event String: The attempt to establish a replication link with
         ......................... DC failed test kccevent
      Starting test: systemlog
         An Error Event occured.  EventID: 0xC0000021
            Time Generated: 12/05/2010   13:24:17
            (Event String could not be retrieved)
         An Error Event occured.  EventID: 0xC0000021
            Time Generated: 12/05/2010   13:24:17
            (Event String could not be retrieved)
         An Error Event occured.  EventID: 0xC0000021
            Time Generated: 12/05/2010   13:24:17
            (Event String could not be retrieved)
         ......................... DC failed test systemlog

   Running enterprise tests on : thesportsclubs.com
      Starting test: Intersite
         ......................... thesportsclubs.com passed test Intersite
      Starting test: FsmoCheck
         Warning: DcGetDcName(GC_SERVER_REQUIRED) call failed, error 1355
         A Global Catalog Server could not be located - All GC's are down.
         Warning: DcGetDcName(TIME_SERVER) call failed, error 1355
         A Time Server could not be located.
         The server holding the PDC role is down.
         Warning: DcGetDcName(GOOD_TIME_SERVER_PREFERRED) call failed, error 135
5
         A Good Time Server could not be located.
         Warning: DcGetDcName(KDC_REQUIRED) call failed, error 1355
         A KDC could not be located - All the KDCs are down.
         ......................... thesportsclubs.com failed test FsmoCheck

C:\Documents and Settings\Administrator>

Did you check the event logs?

Under System:
Dynamic registration or deregistration of one or more DNS records failed because no DNS servers are available.
The PrintQueue Container could not be found because the DNS Domain name could not be retrieved.  Error: 54b

Under Directory Service:
Unable to establish connection with global catalog.

Brief history of recent changes, about 1 week back:

- attempted to establish a trust relationship between the 2000 domain we are having trouble with, and a 2008 domain.  The two networks are connected by VPN.  
- FIrst step was DNS.  Added a secondary forwarder to the 2008 domain and configured the 2008 domain to do the zone transfer.  Then added a stub to the 2008 domain and modified the 2000 domain to do zone transfers.
- attempted to add two way trust using the 2008 wizard.  selected the option for the wizard to do the configs on both sides.  Wizard said it could not do the trusts.  So we stopped, but left the DNS forwarders in place.
-the problem above started after the 2000 DC apparently re-booted after  a power failure.  We then removed the forwarders and zone transfers from each domain.  no change, problem still exists.   We did notice when deleting the stub on the 2008 DC  that the AD integration box was checked .  Didn't intend to do that.

Hope this helps...  
Thanks for your help so far!

Is your DNS service and NetLogon service running on the 2000 DC?

Also, how's your disk space on the 2000 server?

type above... should have read:

- FIrst step was DNS.  Added a secondary forwarder to the 2000 domain and configured the 2008 domain to do the zone transfer.  Then added a stub to the 2008 domain and modified the 2000 domain to do zone transfers.

Is the File Replication Service started?

Is your DNS service and NetLogon service running on the 2000 DC?  Yes, and Yes

Also, how's your disk space on the 2000 server?  2.5GB available on C:  9.4GB on D.  I'd have to ask why, but C and D both have a WinNT folder, D's WinNT  has  a SYSVOL under it.

Is the File Replication Service started?  Yes

Is the File Replication Service started?  Yes