After installing TFS, which installed Sharepoint and hijacked all our IIS stuff, we are trying to set our sites back up. Any sites assigned to an application pool that users v2.0 of the .NET Framework functions properly. Any site using an application pool set to .NET Framework Version v4.0 will not display any formatting from linked CSS files or images. Manually entering the URL to the images or CSS files results in a 500 error. We have ensured that "Static Content" is enabled in the server roles. From there, we are out of ideas.