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FTP 530 Error Access Denied

I run 8 FTP servers behind one firewall and those servers can be accessed globally. There are no firewally rules that specify incoming IP ranges. Recently, one of the 8 FTP servers became inacessible from one location. Attempts to FTP from that location return 530 Access Denied errors. It  is a curious phenonomenon for two reasons:

1. It is only from that one location that a 530 access denied error occurs. From all other locations, you can FTP to the blocked server.
2. From the blocked location, only 1 FTP server is blocked, the other 7 FTP servers are still accessible.

I have spoken to the firewall admin at the location in question and he insists that a 530 error indicates something is blocking the connection on the FTP server side. On the FTP server side however:

1. There are no firewall rules that  distinguish between incoming  IP ranges.

2. The ISP provider of the FTP server network says that the blocking does not emanate from them.

Any ideas?
3 Solutions
What software is running the Servers, IIS or ?
Are they domain named servers or are they ip addressed based (virtual or separate physical servers)?
notcjwAuthor Commented:
The servers are running IIS.
They are Microsoft Virtual 2005 servers running Server 2003.
Each server has a dedicated IP.
530 means the server rejected the user authentication.  Probably accidentally change the password or just mistyped it.  

If it is a secure FTPS site using a client certificate for authenticaiton perhaps the server has the wrong cert loaded for this user.
the 530 is fired by the FTP server when an FTP user autentication fails;
it is not affected by Firewall configurations...
I created a video in You Tube that can walk you through this. I almost pulled the rest of my hair out trying to figure this out so I hope it helps some people.

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