You do not have permissions to open this file on Excel Services.

Hi, I have SharePoint 2007 server farm
I have created 125 site collection on port 80.
Now on single site collection I am getting error while opening Excel 2007 file, below is the error:
Access Denied
You do not have permissions to open this file on Excel Services.

Make sure that the file is in an Excel Services trusted location and that you have access to the file.
While in same site collection excel 2003 file is opening and in others site collections are also working fine and excel 2007 file is opening.

What is the issue with that site collection, even yesterday it’s working perfect, while today error is coming.

Kindly help.    
Who is Participating?
Minesh ShahSharePoint & all about itCommented:
Did you check your ISS server web folders to make sure permisisons are setup correctly there?
prdeshpandeAuthor Commented:
Yes, user can see the files and having uploading permission even from admin id getting the same error.
prdeshpandeAuthor Commented:
Thanks for your valuable support
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