I have developed an asp.net v4 web application project in Visual Studio 2010 using a master page with 2 content holders. The main page of the sight has 2 dropdowns in one contentholder and a formview control in the other, which display and edit information from an Access 2007 database. When rendering the page on the local machine with the local .net development server the page displays and works as expected with the master page layout and the content. When I publish the sight to our Windows 2003 R2/SP2 IIS 6 server with the .net framework 4 loaded and configured for the sight to use, the main page will display and function correctly,, but the master page layout is not loaded into the page. There is no error displayed, and I have tried creating a new aspx with some simple content with the master page and the same scenario, the page displays the master page content correctly on the local asp server but the not on the IIS server when published. Any ideas how to correct or a suggested workaround?