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SMTPRelayToTiRg Queue in Exchange 2010 not forwarding to Exchange 2003

We have added an Exchange 2010 Server to our existing 2003 setup.  It is setup as Mailbox, client Access, and Hub Transport.  We moved several mailboxes over and for a couple days everything ran OK.  Now users with mailboxes on 2010 are not able to send to users on 2003.  The error in the queue is:

The 'Remote delivery' queue (MACFEXC\2221) on server MACFEXC is in retry status. Number of message(s) in the queue: 64. Last error information: 451 4.4.0 Primary target IP address responded with: "500 5.3.3 Unrecognized command." Attempted failover to alternate host, but that did not succeed. Either there are no alternate hosts, or delivery failed to all alternate hosts.

We have deleted the routing connector and recreated, but that hasn't had any effect.

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8/22/2022 - Mon

Create new Send connectors and Receive connectors on exchange 2010 server.

The problem with this error turned out to be that the IP address of the 2010 Hub Transport was entered in the relay section of the 2003 STMP VS as an authorized relay.  Removing is got rid of this problem, and I started getting 451 4.4.0 Primary target IP address responded with: "451 5.7.3 Cannot achieve Exchange Server Authentication."  I traced that to a GPO local security policy, and mail started transferring for a few hours.  Now it back to the Cannot achieve Exchange Server Authentication.  

Enable TLS and Exchange Server authentication check boxes both sides.
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I deleted default receive connector and recreated.