We've just upgraded our server from Windows 2000 Server to Windows 2008 Server 64-bit and copied the .NET 2005 web services across to the new server.
We've still kept the old server and it's response times are always fast.
The new server's first response is slow and then fast subsequently but if not used for a couple of minutes resort to first response (slow) mode.
I've looked into (this (Q_27241923)
) (application pool) solution but I can't get it to work.