Error 500

Sometimes when I browse around my site the main CSS doen't load. I've installed Fiddler and discovered that its producing an error 500. The issue is with my master.css file (custom CSS)

Is this an issue with Sahrepoint os IIS? Can some help me resolve my issue?

I've attached the code I use to append the CSS files in the Master Page.

Thanks
<SharePoint:CssRegistration name="/Style Library/7845/css/sharepoint.css" After="/Style Library/7845/css/master.css" runat="server"/>
	<SharePoint:CssRegistration name="/Style Library/7845/css/master.css" After="/Style Library/7845/css/default.uni-form.css" runat="server"/>
	<SharePoint:CssRegistration name="/Style Library/7845/css/default.uni-form.css" After="/Style Library/7845/css/uni-form.css" runat="server"/>	
	<SharePoint:CssRegistration name="/Style Library/7845/css/uni-form.css" After="/Style Library/7845/css/reset.css" runat="server"/>
	<SharePoint:CssRegistration name="/Style Library/7845/css/reset.css" After="/Style Library/7845/css/rtf.cs" runat="server"/>
	<SharePoint:CssRegistration name="/Style Library/7845/css/rtf.css" After="corev4.css" runat="server"/>

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pokeycamAsked:
Who is Participating?
 
pramodsk40Commented:
Make sure your custom css i.e master.css is published....
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pokeycamAuthor Commented:
I've turned error reporting on and this is what I get in the log file
Url http://7845.net.au:80/Style Library/7845/css/master.css 
App Pool SharePoint - 80 
Authentication NOT_AVAILABLE 
User from token  
Activity ID {00000000-0000-0000-AD12-0080000000ED}

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kukdaiCommented:
try disabling anonymous access if enabled and check back
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