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How do I identify the issue causing file and printer sharing slowness on a Windows 2003 SP2 Server?

One of our Windows 2003 servers is sharing files and printers. Both Windows XP and Windows 7 users are having a hard time opening files and printing from that server. If they drag the file to their desktops it transfers fast and can be opened without issues. We have about 50 concurrent users accessing files on the server during the busiest times of the day.

The server sharing these files also acts as a secondary domain controller. We were having issues about 3 months ago with the hardware and migrated the machine to an ESXi server. It was migrated as a copy of the original server and once we booted it up it was working normally as if nothing had happened. Ever since then we've been having multiple complaints that opening files from that server is taking so long that sometimes the file is never loaded and opened. Copying, pasting, and pinging show no issues with connection.
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Sajid Shaik M
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Hi Guys.  Apologies on this abandoned question.  The individual responsible for this question & project is no longer with the company and the client is no longer active.  Thank you for your responses.