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RDP through VPN

I have installed a new Server 2008 R2 at a clients, to replace a Server 2000 Terminal Server. All local clients can RDP into the new server. Only Administrators are able to RDP into the new server through the VPN. Remote users are getting an access denied message after establishing a VPN connection and attempting to RDP into the new server.
All users are assigned to the domain's Remote Operators group. The CAL licenses have been installed on the new server.
2 Solutions
I'm not familiar with a Remote Operators Group.  Do you mean Remote Desktop Users Group?  There should be no one in the domain built-in "Remote Desktop Users Group" unless you want people to be able to remote into your domain controller.  You need to add the users to the Remote Desktop Users group of the RDS (Remote Desktop Services) server you set up.  Since you are in a domain I suggest creating a group called Remote Desktop Services Access and add this group to the Remote Desktop Users group on the server.  Then add the users to the new group.

Oh and one more thing, are the remote users actually logging on as their domain credentials?  Like if your domain is Contoso and name is john smith they need to enter contoso\jsmith to logon using the correct credentials.

Also check a user account that is not working.  Go to properties of that user, select the "Remote Desktop Services Profile" tab and make sure the "Deny this user permissions to log on to Remote Desktop Session Host" is unchecked.
Maybe you need to add in the new RDP server, open Server Admin Console, the Configuration - Local Users and computers - Groups - Find RDP users or remote users group and add the domain\Remote Operators group to the local RDP group.

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