SharePoint 2010 500 INTERNAL SERVER ERROR

I was working on a permissions issue for a user and when I edited a permission for a user, the site went down and now it just says 500 INTERNAL SERVER ERROR.  Please advise.  

I've turned up the diagnostic logging to error/verbose for all services, but I can't decipher the problem.  The log file is attached

Help!
FC-SPWEB-02-20111025-1206.log
e90mdreiAsked:
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TehzarCommented:
Maybe it can be  resolved by re-inheriting the permission levels on the broken site after you have broken them.  Try this... to reiterate the full steps for resolving both the permissions and 500 Internal Server error, are as follows:

From your broken site, go to the '/_layouts/role.aspx' page, then select 'Edit Permission Levels'

Next, from the same page, click the 'Inherit Permission Levels' button.  

Your site should no longer display the 500 Internal Server error nor should you no longer be able to edit permissions.

 
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TehzarCommented:
From my experience in the past, it was a service account password that expired.  Check your service accounts in AD, see if the accounts are Disabled. 500 error are tricky.  How does your App Logs look?  Any accts failing in Security?  Has anything changed in your environment?  Check the 12 hive log files.  Something may show up there.  Best of luck

ZaR

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e90mdreiAuthor Commented:
app logs are not showing much.  service accounts are not disabled nor is the password expired.  I have two site collections on the same database with the same service accounts.  So I'm assuming that if it's a service account issue, then it would be affecting the other site as well.  Any other thoughts?  
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TehzarCommented:
What specifically does the Error message say?  Does it read "Error Code: 500 Internal Server Error. The request was rejected by the HTTP filter. Contact the server administrator."
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e90mdreiAuthor Commented:
No, just "500 INTERNAL SERVER ERROR"  

Mike
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e90mdreiAuthor Commented:
I can't get to the site to go into edit permission levels.  I'm getting the 500 INTERNAL SERVER ERROR message from the top level site.  
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