DataGridView, Set up columns, Populate Data Table, Bind, but not using columns created in code?

I'm using VB.net 2005.
I have working programs that I populate DataGridViews with something like the following:
Public dt As DataTable
' Call BuildDataTable in Form_Load
Private Sub BuildDataTable()
        dt.Columns.Add("Que#", Type.GetType("System.Int16"))
        dt.Columns.Add("TrimCat#", Type.GetType("System.String"))
        dt.Columns.Add("Lockside", Type.GetType("System.String"))
End Sub
Private Sub FillDataTable()
        Dim QueRow As DataRow
      'Query SQL server table here,  Rdr = cmd.ExecuteReader(), and loop/populate DataTable...
          Do While Rdr.Read() = True ' This automatically advances records until EOF
                QueRow = dt.NewRow
                QueRow.Item("Que#") = Rdr.Item("Queue#")
                QueRow.Item("TrimCat#") = Rdr.Item("TrimCat#")
                QueRow.Item("Lockside") = RTrim(Rdr.Item("LockSideRabbet"))
                dt.Rows.Add(QueRow)
            Loop
End Sub
 ' Bind DataGridView to DataSource
 dgvQueue.DataSource = dt

So the above works just fine.  It automatically creates the colums and populates them with the data.  Here's my new scenerio / question.  I want to have more control over the columns and make one of them a DataGridViewButtonColumn (in code, not in GUI).  So I added code as follows:

Form_Load
        c1 = New DataGridViewTextBoxColumn()
        c1.Name = "Que##"
        Me.dgvQueue.Columns.Add(c1)
        c2 =  New DataGridViewButtonColumn()
        c2.Name = "TrimCat"
        c2.Text = "Click Here"
        c2.HeaderText = "Status-btn"
        c2.UseColumnTextForButtonValue = True
        Me.dgvQueue.Columns.Add(c2)
        c3 = New DataGridViewTextBoxColumn()
        c3.Name = "Lock"
        Me.dgvQueue.Columns.Add(c3)
End Sub

So now when it runs I get the the 3 colums/fields I added in Form_Load (and they are empty) followed by the original 3 fields I built from DataTable.  What I was hoping would happen was the 3 fields/columns from the DataTable would populate the columns I created in Form_Load.

My questions:   Is there a simple way to make the columns I created in Form_Load populate with the data from the DataTable?
Or maybe I am going about this wrong.  Instead of populating the DateTable with my query results in the loop and then binding the DataTable to the DataGridView,  instead should I be populating the DataGridView direclty and not use a DataTable?  (Not sure how to do that.)

Code samples please...

Thanks