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IIS7 - 500 Internal Service - can't read config Error Help!

Hello all,

I had a everyone share on my wwwroot with a asp.net web app on IIS7.  Once I took off the wwwroot everyone share and just added my user account that along with the IIS_ISRS account I added and network service it is still throwing the 500 internal server error.  It has anonymous auth enabled and basic and windows is disabled.  I enabled basic auth as well.  The error is coming up:

can't read configuration file so the web.config of course.  What am I doing wrong?
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