Create .ISO file from a USB boot flash drive

I was given a USB flash drive that is bootable to place an image on a computer. I need to make an ISO file of the USB drive. I went to copy the files as is and create the ISO, but when I attempt to boot from the ISO from my virtual machine it does not recgonize the ISO as bootable.

How do I make the same bootable USB flash drive into an ISO file that makes it recgonize as a bootable ISO?
Who is Participating?
Raymond PengConnect With a Mentor Systems EngineerCommented:
Download imgburn here:

Copy the files off the Flash drive to somewhere on you HD.
Launch imgburn and follow steps below.
motnahp00Connect With a Mentor Commented:
MagicISO works too.
gregurlAuthor Commented:
I never got my ISO to work from the USB for some reason. I was able to create the ISO but it never booted. To solve my issue, the iso had a GHOST image i was really trying to boot up to. So, I booted into a regular virtual machine and placed the image on another drive. I then de-attached the drive and made that the c drive and I got my image down on the virtual machine. I would consider this a workaround to my real issue.
gregurlAuthor Commented:
I do find the two solutions as valid for creating an ISO of my original question.
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