Exchange mailbox access stopped

I have managed to put myself in a bit of a hole last night and need some urgent answers.

I was trying to resolve an activesync issue on a new Excahnge 2010 install and have somehow prevented any access to mailboxes from any client - desktop or web.

The OWA interface gives an LDAP error.
The MAPI is 'unable to connect'

Also I am not able to connect via RDC and have to use vsphere console. I can still get to the event logs via Computer Management on the DC.

I was trying to remove the Activesync virtual directory in order to restore to test for incorrect permissions - as per related articles, but was having issues with the syntax. On looking through IIS manager at the activesync directory I mistakenly deleted the virtual directory through the IIS.

I am not aware of any other changes.

There are no log file errors, and the BPA comes back clean.

I have already migrated some users to the new environment ( The organisation has changed name/domain etc. - hence new server), and they will be unable to access emails this morning.

According to testexchangeconnectivity.com the server is still processing email requests.

I do not know where else to look. Helps or hints hugely appreciated.