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Sharing out IIS folders causing errors

We have recently migrated our old 2003 webserver to a windows 2008 R2 on VMWare for some of out static sites. Before I was able to access the file locations remotely through a share with a UNC path, but for some reason when I attempt to share out one of these folders in windows 2008 RS and IIS 7.5, somehow all my websites throw 401 errors. It seems that the IUSR is being removed for some reason but I am unable to add it back.

The only way I was able to get the sites back on line was to create a new directory structure, move the files and re-point all the apps to the new file locations.

Does anyone have any idea what is going on here and how I can can get around this. I really need to bea ble to access these files remotely so I can use my tools.
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Frederic Sune
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IIS 7/7.5 does not use i_user account anymore.
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Thanks, itinmind. How do I action the last part?
nevermind - I got it
Thanks! that did the trick!