For the most part, instantly; your VMs if currently running will not shut down, but if you restart them or power them down, you won't be able to turn them back on. If you have a "free" license, you can
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Teach the user how to join ESXi hosts to Active Directory domains
Open vSphere Client: Join ESXi host to AD domain: Verify ESXi computer account in AD: Configure permissions for domain user in ESXi: Test domain user login to ESXi host: