I have a web application with 3 site collections. I created a test site collection not related to existing collections. I enabled Anonymous Access in Central Administration. Then, using Site settings, I applied it to my entire test web site. Yet, when browsing test site, the NT login prompt appears asking for login. If I click cancel I get access denied 401 page.
Then I created new web application (on the same farm) with main site collection, changed settings mentioned above and was able to use new site without logging in.
I need to have test site Anonymous Access. What could be different between web application settings that prompts test site users to logging?