Network performance monitoring

I'm looking for a solution to a network performance issue. I have a site where users are complaining about response time. I've relocated one of the users to a different site (physical building) along with their computer, phone and all peripherals to find out if response time improves which it has. I'm having this person remain at the alternative site for a week to continue with the test, which I initially thought would either prove of dis-prove that the network was the issue and not the application or the database. So far, as stated above, performance has noticeable improved according to the user but I'm guessing that I will need more proof than just the users observation and experience to bring to the network team. This is why I'm looking for suggestions that I might use to support my case.

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Download times of large files from a remote site use this as a base line.  How are the sites connected to each other could have a user over there streaming videos, slow switch, etc...  Also I would get more specifics from the users on what is slow.
Do you have a topology diagram of the network?

Just for clarification, users are trying to access a database/file server on the network in the same physical building but get better speeds when accessing the same server remotely?
bjbrownAuthor Commented:
Thanks for the responses, I'm probably not describing this clearly. The users that are having the response time issue are in one building that is located, let's say on our campus. They all hit the same application and same database. I've moved one of the users to a different build on the same campus and this user will continue to access the same application and same database but via a different switch.
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All users in each building connect to one switch and then those switches connect to one central building?  Sounds like a bad switch or port on the one they all connect to.
Or maybe stp
Can you give us a network diagram showing the two locations?  Connection between? number of users are the remote site,  Make/Model of switches involved?  This will help us in determining any potential bottlenecks as well as helping us identify the possible troubleshooting tools to use with switches.

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