Application generated email not being received by recipients

My accounts team use an accounting package that generate emails and send via SMTP.
The software was configured with the IP address of our exchange 2003 server.
This server has been removed and replaced with a new exchange 2010 server.
The software's config was updated with the new IP of the new server.

Since the change, recipients are not receiving emails generated.
The application's support team have confirmed emails sent by the software have left their software and the SMTP is replying with a success message and will not provide us any more assistance as they claim this is a fault with our server.

We have other applications that send emails, they are all working without issue.

Where do I begin to troubleshoot this issue, I would agree with their support team that it is an issue with our Exchange 2010 server but I do not know what logs to read, or settings to change.

Any help would be greatly appreciated.
antoniokingAsked:
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AmitIT ArchitectCommented:
It is due to Exchange 2010. You need to setup a relay connector for your application server. Then email will work
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achaldaveCommented:
How your applications are configured to relay through SMTP? SMTP with authentications or anonymous SMTP connection. If your application can't use authentication, you need to configure anonymous receive connector on exchange.
http://technet.microsoft.com/en-us/library/bb232021%28v=exchg.141%29.aspx

You can start with SMTP logs.

http://blog.globalpointllc.com/exchange-server-2/exchange-2010/logging-smtp-protocol-activity-exchange-2010/
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antoniokingAuthor Commented:
I have an anonymous relay enabled,
SMTP logging is enabled and after sending emails via the application I cannot see anything in the logs referencing the email supposedly sent.

How frequent should the log be updating, is it real time?
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achaldaveCommented:
SMTP logs are real time, if you are not seeing anything in logs means your application is not connecting to exchange, do you have any firewall blocking smtp port between application server and exchange?
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antoniokingAuthor Commented:
Nope application is on a server connected to the same domain as the exchange server and the windiws firewall is off when connected to the domain (GPO)
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antoniokingAuthor Commented:
There was a change needed in a configuration of the actual application.
Thank you all, for all your help.

Antonio
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