Exchange 2010 Mailbox access & Routing issue

Dear Experts.
We are facing strange issue with our exchange 2010 environment,in a day some users are not able to connect to exchange server outlook shows reconnecting at the same time same user cannot access to OWA as well its happening for 1-2 hours after that it will be fine, and it not all users at same time and some times even internal mails are taking long time to deliver.

Exchange environment:
Two exchange 2010 SP3 servers configured as DAG, and CAS array pointing to Node1 and node2 is a cold backup, ESET email security installed on both servers.

Who is Participating?
emtechadminConnect With a Mentor Author Commented:
got support from Microsoft, issue with NOD32 antivirus once i uninstalled it the issue fixed
Gareth GudgerCommented:
Good afternoon,

Anything showing up in the Event Logs?

Are these physical or virtual machines?

Any indication of network issues (bad NIC, cabling, etc).
Iradat SiddiquiCommented:
What exact error the users are getting when they tried login to OWA? Please share how transport role is configured as you are telling some issues with transmitting the mails? Are the internal users able to login to OWA all the time?
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Did you Check event viewer may be some DC/GC issues  .

Can you check connectivity between Mailbox Server & CAS servers

Are you see any errors
Diwakar SharmaCommented:
The problem is looking like with CAS as in Exchange 2010 MAPI and OWA both connects with client access server.

You can use remote connectivity analyzer to troubleshoot this issue.
emtechadminAuthor Commented:
got support from Microsoft, issue with NOD32 antivirus once i uninstalled it the issue fixed
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