I've had this happen a couple of times now... I'm running an IIS 7.0 web server on a couple of different computers (on two different hosting environments) that's serving .asp's with SQL Server connections. At one point, IIS stops allowing connections for about 5 minutes.
On the last such event, I was remoted into the server with RDP and could logon to the web site, access pages, and do everything as normal. However, I tried to log on to the system from another laptop and it timed out. I had the Task Manager open and the system was hardly working (99% idle, 80% memory, networking < 1%, etc.)
There was nothing of note in any of the Windows Event logs and nothing in any of my application logs. No application pools or web sites were stopped.
I'm wondering if any of the IIS settings would have an effect on this... positive or negative. Does anyone know what could be going on or have anything to try? I'm out of ideas!