#5.7.1 smtp;550 5.7.1 Unable to relay

Your message did not reach some or all of the intended recipients.
      Subject:  Accepted: Gdasdsad
      Sent:     4/17/2014 3:04 PM
The following recipient(s) cannot be reached:
      ######,##### on 4/17/2014 3:06 PM
            You do not have permission to send to this recipient.  For assistance, contact your system administrator.
            <Servername #5.7.1 smtp;550 5.7.1 Unable to relay for Destinary email>

So im having this problem for many month and it goes and comes to all users in the exchange server.
I check all the relay setting on the virtual SMTP server, we add the domain, the server ip, the IP of the computer of the user and nothing.
We only used exchange mode in outlook, the problem happen for other emails account and from OWA too.
Today i had it, what i did restart the server, delete all ips in the SMTP virtual server and add it again and it work, but i know it will stop again.
Maybe exchange goes crazy and forgets the relay setting, i really don't know what can be the problem.
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Jeffrey Kane - TechSoEasyConnect With a Mentor Principal ConsultantCommented:
Exchange does NOT forget the setting.  You should NOT have anything listed in the "allow" list of computers.  Rather, you should just have the box checked that says, "Allow all computers which successfully authenticate to relay, regardless of the list above"

Relay Settings Exchange 2003
After you do this, rerun the Configure Email and Internet Connection Wizard to make sure all other settings are correct.
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