1. Check Group Policy Object is mapped correctly to the OU or not.
2.Cross verify GPo is enabled or Disabled
3. Check the inheritance GPO
Further troubleshooting please refer : http://www.windowsnetworking.com/articles-tutorials/windows-server-2008/Top-10-Reasons-Why-Group-Policy-Fails-to-Apply-Part1.html
If you use NetScaler you will want to see these guides. The NetScaler How To Guides show administrators how to get NetScaler up and configured by providing instructions for common scenarios and some not so common ones.
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