Cant get computers to join windows domain anymore on server 2008 R2

We have a second domain that is setup and for some reason now we cant get computers to connect to the domain any longer. The server is running server 2008 R2
We have verified dns settings and such and verified all of the network pieces and all seem to be correct but each time we try to join the domain, we get the message The network path was not found...
I can ping both dc's and I can ping the gateway on that domain but just cant get computers to join the domain.
Any ideas on what to check would be appreciated.
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vmichAsked:
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Chad FranksSenior System EngineerCommented:
Go into your services and make sure the 'TCP/IP NetBIOS Helper' service is started and running.
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Liam SomervilleSenior Security ConsultantCommented:
Give this a shot:

1. Ping the domain name from the DC.
2. Ping the domain name from the client.
3. Perform an ipconfig /flushdns and ipconfig /registerdns on the DCs
4. Perform an ipconfig /flushdns on the clients
5. Ensure your clients point to the DCs for DNS
6. Perform a dcdiag /a /fix on the domain controller
7. Check the Active Directory BPA for errors (find this in Server Manager > Roles > Active Directory
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vmichAuthor Commented:
We have done most of what you have mentioned.
I have not done the dcdiag on the dc but will try that. We have been through all of the event viewers and have not found anything that is useful...
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R RCommented:
Check reverse pointer in DNS and also firewall, otherwise check in windows event from time you start joining computer
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Liam SomervilleSenior Security ConsultantCommented:
The majority of the problems I've run into problems with domain joins has been DNS-related. DCDIAG should turn up any other configuration errors. I'm assuming you've rebooted the DCs/clients?
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vmichAuthor Commented:
Yes have rebooted all many times...
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Liam SomervilleSenior Security ConsultantCommented:
You said you have a second domain up. Tell me about the first. Is it still up and running? Are you using services like DHCP on the same network? Does it have the same domain name/IP range? Were the clients you're trying to join to the second domain originally part of the first? Do you use the hosts file for anything?
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akalyan911Technical ConsultantCommented:
Hi VMich,

If you're not able to join the domain may be the below things need to be consider apart of above things..

1) user account should have privileges to join on the domain. if you're using normal user account (not having admin privileges - not part of admin group). As per the Microsoft article, i do remember Normal user account only have privilege to 25 system can be added on domain.
2) if user account having privileges but not having rights to join on domain as per the domain policy, you can't add the computer on domain.
3) Please first check either primary and secondary DNS having entry of your two DC's.
4) check the network setting --> select the IPV6 or IPV4 --> Click on Advance --> DNS --> check the entry of DNS servers.
5) finally check the DNS entries..
TCP-Setting.jpg
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vmichAuthor Commented:
Issue was that some how the Microsoft file and print service was unchecked on both DC's thus nothing could access it or join the domain...
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vmichAuthor Commented:
I found the issue with the Microsoft file and print service not being turned on both dc's
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