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Yes you can!<br />
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However, as of vista scripts are … not the best option (UAC causes issues). Group policies is the better option, and can be applied to groups or OU’s.
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Yes you can!

However, as of vista scripts are … not the best option (UAC causes issues). Group policies is the better option, and can be applied to groups or OU’s.

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Can group policies be applied to groups AND OU’s?
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Can group policies be applied to groups AND OU’s?

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Yes they can <a href="http://technet.microsoft.com/en-us/library/hh147307%28v=ws.10%29.aspx" rel="ugc">http://technet.microsoft.com/en-us/library/hh147307%28v=ws.10%29.aspx</a>
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Yes they can http://technet.microsoft.com/en-us/library/hh147307%28v=ws.10%29.aspx [http://technet.microsoft.com/en-us/library/hh147307%28v=ws.10%29.aspx]

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Once you have the script, create a GPO under User Configuration - Windows Settings - Scripts - Logon (example) and link to OU.
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Once you have the script, create a GPO under User Configuration - Windows Settings - Scripts - Logon (example) and link to OU.

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Why use a script at all, you can do everything you need via GPO's?
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Why use a script at all, you can do everything you need via GPO's?

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Is it possible to create a logon script based on OU and Group membership?
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Is it possible to create a logon script based on OU and Group membership?

The Microsoft Server topic includes all of the legacy versions of the operating system, including the Windows NT 3.1, NT 3.5, NT 4.0 and Windows 2000 and Windows Home Server versions.