Unable to RDP To cloned xenapp servers

I have a set of Server 2008 R2 Xenapp 6.5 vmware VMs that were recently cloned. I am unable to RDP to these servers. The RDP connection appears to open- flashes a black screen- then closes immediately before asking for credentials with no error message or event log entry on their the source or destination server.  RDP is running on 3389 and I am able to telnet to that port. I validated that the server is able to see licenses from our RDS licensing servers (I even removed and re-added the license servers). The RDP-Tcp listener is set to use any network connection. I also recreated the RDP listener. Windows firewall is not running on the target server.  Citrix/ICA sessions to the servers work just fine.

 Any help would be appreciated.
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jfortunAsked:
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Chris HInfrastructure ManagerCommented:
http://www.experts-exchange.com/OS/Microsoft_Operating_Systems/Q_27614936.html

Although I’m not 100% sure what caused this problem the solution was found by doing the following:
1.      I dispensed with the first clone and created a new server from the OVA created from the source server.
2.      I confirmed port 3398 was listening on this new server but I still could not RDP to it.  I did get presented with a credentials logon box but connection failed with message, “…. No Remote Desktop License Servers available….”
3.      I removed, rebooted then re-added  Remote Desktop Services and still got the same message as above.
4.      I then adjusted RD Session Host Configuration so that, “Restrict each user to a single session” said ‘No’ instead of ‘Yes’.
5.      RDP now worked.
Thank you conradie and dariusg for your suggestions and assistance.
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Chris HInfrastructure ManagerCommented:
Also, have you checked your event logs for errors?  If so, can you post?
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jfortunAuthor Commented:
I did check the event log and there is nothing there. I had checked out the linked answer but my scenario is different- I have confirmed the server is listening on port 3389 as I can telnet to it. I am also not getting any errors regarding RDS Licensing- i am able to run the RDS licensing diagnosis and see plenty of available licenses.  

New symptoms: just now, while I was validating the "Restrict each user to a single session fix" i think i found a solution though I can't think of why this would work.  All I did was logon via the vmware console once with my active directory credentials (not with the local admin credentials) and now RDP is working. I was able to replicate this on 6 of my newly cloned servers.  I then restarted the servers and RDP failed again (with the flashing black screen and no error).  Logging on and off again via the console restored RDP access.
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