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can you explain exactly what the setup is? Where is the USB drive connected to? Another server?<br />
<br />
Have you rebooted the server that has the USB drive attached since you reconnected it?
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can you explain exactly what the setup is? Where is the USB drive connected to? Another server?

Have you rebooted the server that has the USB drive attached since you reconnected it?

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The USB drive is on the same server.<br />
<br />
The server was rebooted. Following the reboot, the iscsi virtual disk does not show up anymore. Import of the existing virtual disk fails with the following error:<br />
<br />
Unable to import the virtual disk. A virtual disk with that file path already exists.
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The USB drive is on the same server.

The server was rebooted. Following the reboot, the iscsi virtual disk does not show up anymore. Import of the existing virtual disk fails with the following error:

Unable to import the virtual disk. A virtual disk with that file path already exists.

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iscsi target is on adata 2TB portable USB drive<br />
It worked initially upon initial setup<br />
The portable drive was disconnected and reconnected<br />
Following re-connection, iscsi target shows status of Error<br />
iscsi initiator shows connected<br />
The iscsi drive does not show up in disk management anymore<br />
iscsi initiator is a hyper-v guest
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iscsi target is on adata 2TB portable USB drive
It worked initially upon initial setup
The portable drive was disconnected and reconnected
Following re-connection, iscsi target shows status of Error
iscsi initiator shows connected
The iscsi drive does not show up in disk management anymore
iscsi initiator is a hyper-v guest

iSCSI in Windows 2012 has status of Error

The Microsoft Legacy Operating System topic includes legacy versions of Microsoft operating systems prior to Windows 2000: All versions of MS-DOS and other versions developed for specific manufacturers and Windows 3/3.1, Windows 95 and Windows 98, plus any other Windows-related versions, and Windows Mobile.

Microsoft Legacy OS

Hyper-V is a native hypervisor; it can create virtual machines on x86-64 systems and supersedes Windows Virtual PC as the hardware virtualization component of the client editions of Windows NT. A server computer running Hyper-V can be configured to expose individual virtual machines to one or more networks. Hyper-V Server supports remote access via Remote Desktop Connection. Administration and configuration of the host OS and the guest virtual machines is generally done over the network.