Authentication issue browsing to network file server

I am having a weird issue where my File Server randomly looses its ability to validate users and provide access to its network shared folders.

Users get a "You do not have permission to access \\xxxx\xxxxx. Contact your network administrator to request access".  Oddly enough, these users have access to the folders and was working leading up to logging in this morning.

This issue occurred on Friday after a restart of the server and again this morning (no restart this time).  Both times it has taken me to remove the server from the Active Directory domain and re-join.  Post re-joining, access is restored.

Attached is a before and after screen shot of the folder permissions screen as well as the error the users received.  I also received the error when browsing from the Domain Controller across for shared folders.  I was able to manage server items using Computer Manager file.

Running Server 2012 Core on the file server on VMWare infrastructure.  Need to get this sorted asap as the guys at this site are 2 hours ahead of me and the issue has occurred at the start of their working day.
BEFORE-Error.JPG
BEFORE-Folder-Permissions.JPG
AFTER-Folder-Permissions.JPG
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ddawesAsked:
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ddawesConnect With a Mentor Author Commented:
A setting was set in VMWare to allow "Passthrough".  When this was unset and computers restarted, this error seems to have gone away.  So far we are at 4 days and no issues.
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ddawesAuthor Commented:
It has just happened for a second time today.  Issue seems to be getting worse. :| Any ideas what might be causing this?

Thinking I might start the process to build a replacement VM and migrate data off the old one.
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Praveen Kumar BonalaProgrammer AnalystCommented:
Please check Logs on the server,
we will get the clues, and it will be useful to identify the reason.
Kindly check and provide the log.
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ddawesAuthor Commented:
I had already checked the logs and nothing out of the ordinary is there.    I will check them again once I reach the office, but I suspect that I won't find anything.
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Praveen Kumar BonalaProgrammer AnalystCommented:
Please check Security and System logs.
If your server denying access mean defiantly Audit failure log should be received.
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ddawesAuthor Commented:
The answer provided resolved the issue.
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