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How to use windows server as a 2 factor radius server for security appliance

We have a security appliance we host which provides secure connection to remote servers and workstations.      We would like to implement 2 factor authentication as one of our clients requires it.     I contacted the security appliance support and they indicated that using a Radius server with 2 factory authentication enabled would meet this requirement.

Can I use a windows server for the radius server?
Does this need to be on one of our domain controllers?
Will this effect our internal user authentications?
Are there guides available for this setup?

Thanks
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Can I use a windows server for the radius server?
Yes
Does this need to be on one of our domain controllers?
NO
Will this effect our internal user authentications?
No
Are there guides available for this setup?
Yes
http://www.freeccnaworkbook.com/blog/ccna-security/cisco-ios-radius-authentication-with-windows-server-2012-nps

http://www.juniper.net/documentation/en_US/junos14.2/topics/task/configuration/radius-authentication-configuring.html

http://blog.pluralsight.com/using-radius-for-authentication

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Commented:
Thank you for the guides on setup of the Radius.    I don't believe however these are 2 factor authentication setups?
I don't believe however these are 2 factor authentication setups?
Why not? Are you thinking of dropping the use of the "security appliance" and relying exclusively on the Radius service? Is it because the combination of 'security appliance + Radius service' doesn't strictly rely on "something you have"? Some other reason?

Tom