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Can you try with the following change?<br />
<br />
OpenWebConfiguration(&quot;./WE<wbr />BUI&quot;)
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Can you try with the following change?

OpenWebConfiguration("./WEBUI")

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I could solve the problem by giving &nbsp; my web application name ( &quot;/ App name&quot;) &nbsp;hosted in iis in open configuration.
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I could solve the problem by giving   my web application name ( "/ App name")  hosted in iis in open configuration.

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I can read connection string from webconfig file &nbsp;from visual studio from the below mentioned code<br />
in login.aspx page<br />
<br />
&nbsp;Private constr As String<br />
&nbsp; &nbsp; Private rootWebConfig As System.Configuration.Confi<wbr />guration<br />
&nbsp; &nbsp; Private connString As System.Configuration.Conne<wbr />ctionStrin<wbr />gSettings<br />
<br />
rootWebConfig = System.Web.Configuration.W<wbr />ebConfigur<wbr />ationManag<wbr />er.OpenWeb<wbr />Configurat<wbr />ion(&quot;/WEBU<wbr />I&quot;) &nbsp; &nbsp;where WEBUI is project folder where webconfig resides.<br />
<br />
&nbsp; &nbsp; &nbsp; &nbsp; connString = rootWebConfig.ConnectionSt<wbr />rings.Conn<wbr />ectionStri<wbr />ngs(&quot;Sqlse<wbr />rver&quot;)<br />
&nbsp; &nbsp; &nbsp; &nbsp; constr = connString.ConnectionStrin<wbr />g<br />
<br />
I created &nbsp;web deploy package and imported into localhost IIS 7. import application worked fine. when I tried to browse the login.aspx from IIS published folder I get the following msg<br />
<br />
Server Error in '/KHWEB' Application. &nbsp;- &nbsp;KHWEB &nbsp;web application name in IIS7<br />
<br />
Object reference not set to an instance of an object.
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I can read connection string from webconfig file  from visual studio from the below mentioned code
in login.aspx page

 Private constr As String
    Private rootWebConfig As System.Configuration.Configuration
    Private connString As System.Configuration.ConnectionStringSettings

rootWebConfig = System.Web.Configuration.WebConfigurationManager.OpenWebConfiguration("/WEBUI")    where WEBUI is project folder where webconfig resides.

        connString = rootWebConfig.ConnectionStrings.ConnectionStrings("Sqlserver")
        constr = connString.ConnectionString

I created  web deploy package and imported into localhost IIS 7. import application worked fine. when I tried to browse the login.aspx from IIS published folder I get the following msg

Server Error in '/KHWEB' Application.  -  KHWEB  web application name in IIS7

Object reference not set to an instance of an object.

Reading from web configuration file

The successor to Active Server Pages, ASP.NET websites utilize the .NET framework to produce dynamic, data and content-driven web applications and services. ASP.NET code can be written using any .NET supported language. As of 2009, ASP.NET can also apply the Model-View-Controller (MVC) pattern to web applications

ASP.NET

IIS is Internet Information Services, the web server included with Windows Server operating systems. All current versions are built on a modular architecture; modules can be added or removed individually so that those required for specific functionality are installed. The full installation of IIS includes HTTP, security, content, compression, caching, logging and diagnostics.