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exchange 2013 accepted domain issue

We have put a domain into a new install of exchange server 2013. It so transpires that we have to use this domain on a different network so we have deleted the domain from accepted domains. Also removed addresses on mailboxes and checked the domain had been removed with ADSI edit.
But now we try to send to this domain we get it stuck in queue with 4.4.1 error encountered with primary target IP address

Any ideas?
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Did you checked send connector settings. Check DNS also. It seems you are unable to resolve it.
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Can ping the domains
Are you able to do telnet to that domain on Port 25. Possible chances it might be blocked via firewall.
It is receiving emails from everyone else except our new domain.
I believe it is a problem because we put it into exchange 2013
Test using telnet. Use telnet to submit an email and share the result.
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Murali Reddy
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