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Unable to access SMB Share over VPN

I have a customer with about 10 locations.  For some reason although the VPN's have been setup for years.  A server at one time cannot be access over the VPN all of a sudden.  

Everything works fine internally but it will not allow any user from any site to browse remotely either through the  NetBIOS name, DNS Name, or IP Address.

When trying to browse I get the error Path Not found.... 0x80070035.


I have tried rebooting the server, clients, other servers and a few articles I found searching with no luck.
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I can ping their server by name and IP and everything else on the VPN. And the local server can browse across the network and local clients can browse the server but no one from across the VPN
Windows firewall enabled?
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Still an issue?

worth running a trace route to confirm its not a routing issue over the VPN, but I agree that firewall is probably the first thing to rule out. Can you try anything ele (telnet, http, https, dns ) to the server to confirm if any traffic is flowing or just SMB has a problem?