Exchange 2010, unable to relay 550 5.7.1

Dear Experts,

I run an on-premise Exchange 2010 on SBS2011. I have not been successful in configuring the Receive Connector, Unable to receive emails from internal users and external users. comes with messages "The error that the other server returned was:
550 5.7.1 Unable to relay"

I have Re Created all the receive connection as below: please see images.

I still have no luck to receive emails....

any advice please?
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it seems that there is mis configuration of the connectors. Kindly refer below article to configure the permissions and authentication and make sure that port 25 is opened
imkotteesSenior Messaging EngineerCommented:

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