Making syslinux bootable iso's for testing with a vm

I am building a bootable usb that uses syslinux and boots a esxi into a scripted install.
Booting the physical host to test different changes made takes time, because the boot process is slow. Is there any way I could create an iso file that acts similar to a syslinux bootbale usb and that I could boot with a virtual machine from instead?

Preferably I would like to do the task in a Linux command shell.
Would mkisofs do the trick? Seems like fat32 isn't a option there?
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Thomas Zucker-ScharffSolution GuideCommented:
Have you tried gizmo? you only need to install the virtual drive component and the shared components.  This is a windows based program.
itniflAuthor Commented:
Looks like gizmo wants to mount iso's as cd-drives and not usb drives I can write to...
Thomas Zucker-ScharffSolution GuideCommented:
Hmm. Yes forgot about that. Sorry. But if you are testing the iso,  does it make a difference? I use SARDU, which creates either a bootable syslinux based USB or an ISO. Either can be modified using the tools within SARDU. I create am iso then test it by mounting it with gizmo and then either use SARDU or another bootable usb creator to burn the iso to a usb  (universal usb creator also works).

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You can use ESX powercli to assemble ISO and IMG files.
itniflAuthor Commented:
I can make an iso and burn to usb with a tool like those you mentioned or for instance Rufus. However, I was hoping for creating everything in the same way as when I create USB bootables directly. Accepting answers as a good enough solution.
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