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Storage vMotion if you are licensed for it.<br />
<br />
otherwise you will have to do it manually.<br />
<br />
see here<br />
<br />
<a href="https://www.experts-exchange.com/A_9249.html" rel="ugc">HOW TO: Clone or Copy a virtual machine in VMware vSphere Hypervisor ESX/ESXi 4.x or ESXi 5.0</a><br />
<br />
<a href="https://www.experts-exchange.com/Software/VMWare/A_12515.html" rel="ugc">HOW TO: Select the right answer to &quot;I Moved It&quot; or &quot;I Copied It&quot; in VMware vSphere (ESXi)</a>
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Storage vMotion if you are licensed for it.

otherwise you will have to do it manually.

see here

HOW TO: Clone or Copy a virtual machine in VMware vSphere Hypervisor ESX/ESXi 4.x or ESXi 5.0 [https://www.experts-exchange.com/A_9249.html]

HOW TO: Select the right answer to "I Moved It" or "I Copied It" in VMware vSphere (ESXi) [https://www.experts-exchange.com/Software/VMWare/A_12515.html]

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Thanks for the prompt reply. <br />
<br />
However, I wanted to point out that there are about 10 different machines &nbsp;including VMs, ISOs, Templates, etc. in this datastore. &nbsp;<br />
Is there &nbsp;any way I can do a Storage vMotion (yes I have license) &nbsp;for ALL the &nbsp;machines/VMs at once?
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Thanks for the prompt reply.

However, I wanted to point out that there are about 10 different machines  including VMs, ISOs, Templates, etc. in this datastore.  
Is there  any way I can do a Storage vMotion (yes I have license)  for ALL the  machines/VMs at once?

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<div class="content wysiwyg-content">
What is the easiest and best way to move &nbsp;contents from one datastore to another in vSphere 5.5<br />
Thanks.
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What is the easiest and best way to move  contents from one datastore to another in vSphere 5.5
Thanks.

Moving VMs from one Datastore  to another

VMware, a software company founded in 1998,  was one of the first commercially successful companies to offer x86 virtualization. The storage company EMC purchased VMware in 1994. Dell Technologies acquired EMC in 2016. VMware’s parent company is now Dell Technologies.

VMware has many software products that run on desktops, Microsoft Windows, Linux, and macOS, which allows the virtualizing of the x86 architecture.  Its enterprise software hypervisor for servers, VMware vSphere Hypervisor (ESXi), is a bare-metal hypervisor that runs directly on the server hardware and does not require an additional underlying operating system.