The remote server returned an error: (401) Unauthorized. not able to authenticate

Hi,
We are using custom solution that is using IIS regular web server.
I have certificate configured for https://website. it is binded to 443 and working.

Users need to authenticate to the web server using windows authentication https with cert. But I am getting 401 unauthorized error for some users but not for others. In particular users in source are not able to authenticate.
Web server is in the target. But some users from source successfully authenticate. Target users have no problem to authenticate

How do you troubleshoot this issue? Where to look to solve this quickly?

We tried clearing IE cookies, moving workstations to different OUs. The issue is random. Everything worked fine few days ago, and then smth happened.

So now users are continually prompted for creds when accessing webserver url and then authentication fails with 401. Also we saw this error in the event log.

They are not suppose to get prompted at all because the web site is in the Intranet zone.

Error in the event log

failed to set DefaultTargetDNS via GetSetting http call. Error:GetConfigSetting Http call failed - url: https://website/ActivationPortal/Settings/GetConfigSetting, urlParams: configGroup=DomainSettings&keyName=defaultTargetDNS, Error: The remote server returned an error: (401) Unauthorized.,    at System.Net.HttpWebRequest.GetResponse()
   at Msm.PreMigrationTestCommon.HttpHelper.SendHttpRequest(String method, String uri, String parameters, String userName, String domain, String password, String authType)
   at Msm.PreMigrationTestCommon.TestProcessor.GetConfigSetting(String migrationId, String configGroup, String keyName, String context)
creative555Asked:
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giltjrConnect With a Mentor Commented:
The 401 means that either the user-id/password is not valid, could not be validated, or is not authorized.

So, yes, if the server never got a response from the DC a 401 could be issues, but I would assume it they would be sitting there for awhile while IIS waits for a response.

If the issue is resolved, what was the problem.
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giltjrCommented:
The HTTP 401 error normally means that that the user-id/password is not valid.  

It appears the event log entry is from the end-users computer.  You need to look at the server.
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creative555Author Commented:
thank you! The issue was resolved.
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creative555Author Commented:
could it be the issue with DC? Where doesn't get creds from DC?
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creative555Author Commented:
thank you so much! Sorry for the delay
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