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No, I'm afraid not, VDP must be installed on a VMware datastore, so either VMFS or NFS.<br />
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If you Windows server is running NFS, the ESXi host could connect to the NFS export on Windows.
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Andrew,<br />
To clarify, the appliance must be installed on a VMware datastore but i should be able to set up an NFS share on a windows box to the esata?<br />
Thanks
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Can vsphere data protection send back ups to esata devices that are connected to a windows based system.
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Can vsphere data protection send back ups to esata devices that are connected to a windows based system.

Vsdp using esata devices

VMware, a software company founded in 1998,  was one of the first commercially successful companies to offer x86 virtualization. The storage company EMC purchased VMware in 1994. Dell Technologies acquired EMC in 2016. VMware’s parent company is now Dell Technologies.

VMware has many software products that run on desktops, Microsoft Windows, Linux, and macOS, which allows the virtualizing of the x86 architecture.  Its enterprise software hypervisor for servers, VMware vSphere Hypervisor (ESXi), is a bare-metal hypervisor that runs directly on the server hardware and does not require an additional underlying operating system.